ساخت و تعیین ویژگی ذرات نانوکامپوزیتی متخلخل سیلیکای مغناطیسی برای کاربردهای پزشکی و بررسی سمیت سلولی آن‌ها

Background and purpose: One of the most important challenges in nanomedicine is timely diagnosis and effective treatment of chronic disease. The aim of this research was to synthesize and characterize 3-layered magnetic silica nanocomposite with simultaneous application as a contrast agent in magnetic resonance imaging (MRI) and for drug delivery. Materials and methods: In this research, magnetite iron oxide nanoparticles by co-precipitation method, first layer of silica by a method similar to Stober method and second layer (mesoporous silica) by removing template method were produced. Existence or deletion of surfactant molecules by Fourier Transform Infrared (FTIR) spectroscopy, particle size and morphology of particles by high resolution scanning transmission electron microscopy (HR-STEM), magnetic properties of particles by vibrating sample magnetometer (VSM) instrument, mesoporous structure by Brunauer-Emmett-Teller (BET) method and also crystalline structure of nanocomposite by X-Ray diffraction (XRD) method were investigated. Diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays were used to study cytotoxicity of the produced particles. Results: Produced 3-layered nanocomposite had the size of 180 ± 10 nm, specific area and average pore diameter of 390.4m2/gand 3.01 nm, respectively. The saturation magnetization value of nanocomposite was 21 ± 0.5 emu/g. Cell cytotoxicity of nanocomposite with human prostate carcinoma epithelial-like cell line Du-145 and erythroleukemia cells K562 were reviewed. The results revealed that synthesized nanocomposite enhanced no cytotoxicity in tested concentrations. Conclusion: The result of present study illustrated that produced nanocomposite has preliminary essential capability to be used as MR imaging agent and drug delivery system.